Endothelial cell calcium increases during flow-induced dilation in isolated arterioles
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The influence of flow on endothelial intracellular calcium concentration ([Ca2+](i)) was determined in intact, isolated arterioles by selectively loading endothelial cells with the calcium-sensitive fluorescent dye fura-2. A fluorescence microscope coupled to a digital image processor was used to simultaneously measure fura-2 fluorescence and microvessel diameter. Flow through the arteriole significantly increased endothelial [Ca2+](i) and dilated arterioles. Acetylcholine also increased in endothelial [Ca2+](i) and caused vasodilation. In comparison, adenosine did not alter endothelial [Ca2+](i) but dilated arterioles. Removal of the endothelium abolished the responses to flow and acetylcholine but not adenosine. These results provide strong support for the involvement of calcium in endothelium-dependent dilation of isolated arterioles by flow and agonists and emphasize the importance of studying endothelial function in intact vessels.
author list (cited authors)
Falcone, J. C., Kuo, L., & Meininger, G. A.