Enhancement of Anti-tumorigenic Polysaccharide Production, Adhesion, and Branch Formation of Bifidobacterium bifidum BGN4 by Phytic Acid
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The polysaccharide (BB-pol) extracted from Bifidobacterium bifidum BGN4 showed growth inhibitory effects onseveral colon cancer cell lines such as HT-29 and HCT-116. To increase the yield of polysaccharide, B. bifidum BGN4 wascultured in various culture media with different compositions. When B. bifidum BGN4 was cultured in modified MRS brothcontaining phytic acid, the cells showed increased branch formation and enlarged morphology. The content of totalcarbohydrate and the ability of adhesion to intestinal epithelial cells were also increased by phytic acid. The polysaccharideobtained from the cells grown in the presence of phytic acid inhibited the proliferation of cancer cell lines such as HT-29 andMCF-7 cells but not normal colon cell line, FHC. Taken together, Bifidobacterium grown in the presence of phytic acid mayconfer enhanced beneficial function for the host.