Whole-Cell Biocatalysis for Producing Ginsenoside Rd from Rb1 Using Lactobacillus rhamnosus GG. Academic Article uri icon

abstract

  • Ginsenosides are the major active ingredients in ginseng used for human therapeutic plant medicines. One of the most well-known probiotic bacteria among the various strains on the functional food market is Lactobacillus rhamnosus GG. Biocatalytic methods using probiotic enzymes for producing deglycosylated ginsenosides such as Rd have a growing significance in the functional food industry. The addition of 2% cellobiose (w/v) to glucose-free de Man-Rogosa-Sharpe broths notably induced -glucosidase production from L. rhamnosus GG. Enzyme production and activity were optimized at a pH, temperature, and cellobiose concentration of 6.0, 40C, and 2% (w/v), respectively. Under these controlled conditions, -glucosidase production in L. rhamnosus GG was enhanced by 25-fold. Additionally, whole-cell homogenates showed the highest -glucosidase activity when compared with disrupted cell suspensions; the cell disruption step significantly decreased the -glucosidase activity. Based on the optimized enzyme conditions, whole-cell L. rhamnosus GG was successfully used to convert ginsenoside Rb1 into Rd.

published proceedings

  • J Microbiol Biotechnol

author list (cited authors)

  • Ku, S., You, H. J., Park, M. S., & Ji, G. E.

complete list of authors

  • Ku, Seockmo||You, Hyun Ju||Park, Myeong Soo||Ji, Geun Eog

publication date

  • July 2016