Electrophysiology of AChE-positive neurons in basal forebrain slices. Academic Article

abstract

• We have utilized a guinea pig in vitro brain slice preparation of the medial septum (MS) and the vertical and horizontal limbs of the nucleus of the diagonal band of Broca (nDBB) to identify and classify different cell types within cholinergic nuclei. Utilizing a double-labeling technique which pairs intracellular injection of the fluorescent dye Lucifer yellow with acetylcholinesterase (AChE) histochemistry, we were able to correlate electrophysiological characteristics with a specific cholinergic cell marker. We report that at least two cell groups can be identified electrophysiologically within the MS/nDBB complex, and one population of neurons demonstrates distinct electrophysiological characteristics that are highly correlated with positive AChE-staining.

authors

• Griffith, William

published proceedings

• Neurosci Lett

author list (cited authors)

• Griffith, W. H., & Matthews, R. T.

citation count

• 94

complete list of authors

• Griffith, WH||Matthews, RT

publication date

• November 1986

publisher

• Elsevier  Publisher

published in

• NEUROSCIENCE LETTERS  Journal

keywords

• ACETYLCHOLINESTERASE
• Action Potentials
• Animals
• Choline O-Acetyltransferase
• Cholinergic Fibers
• Guinea Pigs
• Histocytochemistry
• In Vitro Techniques
• Limbic System
• Septum Pellucidum

PubMed Central ID

• 3785743

Digital Object Identifier (DOI)

• 10.1016/0304-3940(86)90553-7

start page

• 169

end page

• 174

volume

• 71

issue

• 2

URL

• http://dx.doi.org/10.1016/0304-3940(86)90553-7