Venular endothelial cells from bovine heart. Academic Article

abstract

• Coronary venular endothelial cells were isolated by a bead-perfusion technique that allowed the selection of endothelial cells from venules of a specific size. Culture conditions for the microvascular cells were established. Cells grew well in supplemented Dulbecco's modified Eagle's medium. The effect of various substrata on the proliferation of the venular endothelial cells was determined. Matrigel, gelatin, and fibronectin supported high levels of proliferation. Cell shape was correlated with ability of the substratum to support cell proliferation. Cells exhibiting a broad, flattened morphology achieved high levels of proliferation. The formation of vessel meshworks by the coronary venular endothelial cells provides an in vitro model for the study of coronary angiogenesis. Confluent monolayers of these cells can be utilized to examine mechanisms of water and protein transport across coronary venules.

authors

• Meininger, Cynthia

published proceedings

• Am J Physiol

author list (cited authors)

• Schelling, M. E., Meininger, C. J., Hawker, J. R., & Granger, H. J.

citation count

• 80

complete list of authors

• Schelling, ME||Meininger, CJ||Hawker, JR||Granger, HJ

publication date

• June 1988

publisher

• American Physiological Society  Publisher

published in

• AMERICAN JOURNAL OF PHYSIOLOGY  Journal

keywords

• Animals
• Cattle
• Cell Division
• Cell Separation
• Cells, Cultured
• Coronary Vessels
• Endothelium, Vascular
• Neovascularization, Pathologic
• Perfusion
• Venules

PubMed Central ID

• 2454587

Digital Object Identifier (DOI)

• 10.1152/ajpheart.1988.254.6.H1211

start page

• H1211

end page

• H1217

volume

• 254

issue

• 6 Pt 2

URL

• http://dx.doi.org/10.1152/ajpheart.1988.254.6.h1211