Acute tolerance to ethanol inhibition of NMDA-mediated EPSPs in the CA1 region of the rat hippocampus. Academic Article

abstract

• The time course of ethanol-induced inhibition of NMDA-mediated synaptic activity was studied in brain slices using extracellular electrophysiological techniques in the CA1 region of the hippocampus. Bath application of 60 mM ethanol inhibited NMDA-mediated field excitatory postsynaptic potentials (EPSPs) by at least 45% in 7/11 of the slices tested, with the remaining 4 slices inhibited by 8.7-35%. Most slices inhibited by at least 45% showed a significant reduction in ethanol inhibition over a 15 min ethanol exposure period, suggesting the development of acute tolerance. In a second set of experiments, tolerance to ethanol-induced inhibition of NMDA-mediated EPSPs that developed over time during the first ethanol exposure persisted during a second ethanol exposure. In contrast to ethanol, inhibition of EPSPs by the NMDA antagonist DL-2-amino-5-phosphonopentanoic acid (APV) remained stable during a comparable application of the drug. These results suggest that acute tolerance can develop to ethanol inhibition of NMDA mediated synaptic activity in the hippocampus.

authors

• Griffith, William

published proceedings

• Brain Res

author list (cited authors)

• Grover, C. A., Frye, G. D., & Griffith, W. H.

citation count

• 50

complete list of authors

• Grover, CA||Frye, GD||Griffith, WH

publication date

• April 1994

publisher

• Elsevier  Publisher

published in

• BRAIN RESEARCH  Journal

keywords

• 2-Amino-5-phosphonovalerate
• Animals
• Drug Tolerance
• Ethanol
• Evoked Potentials
• Hippocampus
• In Vitro Techniques
• Male
• N-Methylaspartate
• Rats
• Rats, Sprague-Dawley

PubMed Central ID

• 7913393

Digital Object Identifier (DOI)

• 10.1016/0006-8993(94)90906-7

start page

• 70

end page

• 76

volume

• 642

issue

• 1-2

URL

• http://dx.doi.org/10.1016/0006-8993(94)90906-7