Immortal time: circadian clock properties of rat suprachiasmatic cell lines. Academic Article

abstract

• Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of the SCN in situ. Immortalized SCN cells generated robust rhythms in uptake of the metabolic marker 2-deoxyglucose and in their content of neurotrophins. The phase relationship between these rhythms in vitro was identical to that exhibited by the SCN in vivo. Transplantation of SCN cell lines, but not mesencephalic or fibroblast lines, restored the circadian activity rhythm in arrhythmic, SCN-lesioned rats. Thus, distinctive oscillator, pacemaker, and clock properties of the SCN are not only retained but also maintained in an appropriate circadian phase relationship by immortalized SCN progenitors.

authors

• Earnest, David

published proceedings

• Science

altmetric score

• 3.5

author list (cited authors)

• Earnest, D. J., Liang, F. Q., Ratcliff, M., & Cassone, V. M.

citation count

• 108

complete list of authors

• Earnest, DJ||Liang, FQ||Ratcliff, M||Cassone, VM

publication date

• January 1999

publisher

• American Association for the Advancement of Science (AAAS)  Publisher

published in

• Science  Journal

keywords

• Animals
• Biological Clocks
• Brain-derived Neurotrophic Factor
• Cell Line
• Cell Transplantation
• Circadian Rhythm
• Deoxyglucose
• Glucose-6-Phosphate
• Glycogen
• Graft Survival
• Male
• Motor Activity
• Nerve Growth Factors
• Neurons
• Neurotrophin 3
• Rats
• Rats, Sprague-Dawley
• Stem Cells
• Suprachiasmatic Nucleus

PubMed Central ID

• 9924030

Digital Object Identifier (DOI)

• 10.1126/science.283.5402.693

start page

• 693

end page

• 695

volume

• 283

issue

• 5402

URL

• http://dx.doi.org/10.1126/science.283.5402.693