Genetic transformation of Borrelia burgdorferi. Academic Article

abstract

• The development of robust genetic tools to manipulate Borrelia burgdorferi, the etiologic agent of Lyme disease, now allows investigators to assess the role(s) of individual genes in the context of experimental Lyme borreliosis. This unit is devoted to the description of experimental approaches that are available for the molecular genetic analysis of B. burgdorferi with an emphasis on cultivation, electrotransformation, selection of desired mutants, and genetic complementation of acquired mutants. The intent is to provide a consensus protocol that encapsulates the methodologies currently employed by the B. burgdorferi research community and describe pertinent issues that must be accounted for when working with these pathogenic spirochetal bacteria.

authors

• Hyde, Jenny
• Skare, Jon

published proceedings

• Curr Protoc Microbiol

altmetric score

• 9.008

author list (cited authors)

• Hyde, J. A., Weening, E. H., & Skare, J. T.

citation count

• 22

complete list of authors

• Hyde, Jenny A||Weening, Eric H||Skare, Jon T

publication date

• February 2011

publisher

• Wiley  Publisher

published in

• Current protocols in microbiology  Journal

keywords

• Borrelia Burgdorferi
• Culture Techniques
• Electroporation
• Genetic Complementation Test
• Humans
• Lyme Disease
• Mutation
• Transformation, Genetic

PubMed Central ID

• 21400675

Digital Object Identifier (DOI)

• 10.1002/9780471729259.mc12c04s20

start page

• Unit

end page

• 12C.4

volume

• Chapter 12

issue

• 1

URL

• http://dx.doi.org/10.1002/9780471729259.mc12c04s20