Fluorogenic probes with substitutions at the 2 and 7 positions of cephalosporin are highly BlaC-specific for rapid Mycobacterium tuberculosis detection. Academic Article uri icon


  • Current methods for the detection of Mycobacterium tuberculosis (Mtb) are either time consuming or require expensive instruments and are thus are not suitable for point-of-care diagnosis. The design, synthesis, and evaluation of fluorogenic probes with high specificity for BlaC, a biomarker expressed by Mtb, are described. The fluorogenic probe CDG-3 is based on cephalosporin with substitutions at the 2 and 7positions and it demonstrates over 120,000-fold selectivity for BlaC over TEM-1 Bla, the most common -lactamase. CDG-3 can detect 10 colony-forming units of the attenuated Mycobacterium bovis strain BCG in human sputum in the presence of high levels of contaminating -lactamases expressed by other clinically prevalent bacterial strains. In a trial with 50 clinical samples, CDG-3 detected tuberculosis with 90% sensitivity and 73% specificity relative to Mtb culture within one hour, thus demonstrating its potential as a low-cost point-of-care test for use in resource-limited areas.

published proceedings

  • Angew Chem Int Ed Engl

altmetric score

  • 63.25

author list (cited authors)

  • Cheng, Y., Xie, H., Sule, P., Hassounah, H., Graviss, E. A., Kong, Y., Cirillo, J. D., & Rao, J.

citation count

  • 60

complete list of authors

  • Cheng, Yunfeng||Xie, Hexin||Sule, Preeti||Hassounah, Hany||Graviss, Edward A||Kong, Ying||Cirillo, Jeffrey D||Rao, Jianghong

publication date

  • August 2014