Brain biopsy is essential for accurate diagnosis but is frequently avoided in veterinary medicine because of doubts about its safety, reliability, and clinical value. Data available from human and veterinary investigations suggest that such doubts are largely unwarranted. Many devices are available to guide minimally invasive biopsy but some can be costly to purchase and use, which can be problematic in veterinary medicine. Nowadays, costs can be substantially reduced by using 3-dimensional-printed guides.