Growth and differentiation of PC6 cells: the effects of pulsed electromagnetic fields (PEMF). Academic Article

abstract

• Previous studies in our laboratory showed that neurite outgrowth in vitro and nerve regeneration in vivo were stimulated by 2 Hz, 0.3 mT (3 G) pulsed electromagnetic fields (PEMF). To learn more about the effects of PEMF on nerve cells, we exposed PC6 cells, a standard neuronal-like cell model, to the same pulsed electromagnetic fields for 2 h/day for 2 days and asked whether two different cell processes, proliferation and differentiation, were affected. The cells were also treated with a differentiating agent, nerve growth factor (NGF), to further define any interactive effects. We found that proliferation was unaffected by either PEMF or NGF alone or in combination. Differentiation, expressed as neurite outgrowth, was strongly upregulated with NGF, but this NGF response was significantly depressed in cells treated with PEMF.

authors

• Brandt, Paul

published proceedings

• Bioelectromagnetics

author list (cited authors)

• Shah, J. P., Midkiff, P., Brandt, P. C., & Sisken, B. F.

citation count

• 27

complete list of authors

• Shah, JP||Midkiff, P||Brandt, PC||Sisken, BF

publication date

• May 2001

publisher

• Wiley  Publisher

published in

• Bioelectromagnetics  Journal

keywords

• Animals
• Cell Differentiation
• Cell Division
• Electromagnetic Fields
• Kinetics
• Models, Neurological
• Nerve Growth Factor
• Neurons
• PC12 Cells
• Rats
• Time Factors

PubMed Central ID

• 11298388

Digital Object Identifier (DOI)

• 10.1002/bem.49

start page

• 267

end page

• 271

volume

• 22

issue

• 4

URL

• http://dx.doi.org/10.1002/bem.49