Investigating endothelial invasion and sprouting behavior in three-dimensional collagen matrices. Academic Article

abstract

• Seeding a monolayer of primary human endothelial cells on the surface of a polymerized three-dimensional collagen matrix in the presence of pro-angiogenic stimuli allows manipulation and analysis of rapid sprouting responses. This protocol is useful for elucidating incompletely defined intracellular mechanisms downstream of pro-angiogenic factors that regulate sprout formation and initiation, and can also be used to test the efficacy of pro-and anti-angiogenic compounds. We present protocols to culture endothelial cells, prepare three-dimensional collagen matrices and quantify and image rapid endothelial sprouting responses (24 h). This protocol can be carried out using either type I or type II collagen matrices with primary endothelial cells isolated from macrovascular and microvascular sources of varying species.

authors

• Bayless, Kayla

published proceedings

• Nat Protoc

author list (cited authors)

• Bayless, K. J., Kwak, H., & Su, S.

citation count

• 83

complete list of authors

• Bayless, Kayla J||Kwak, Hyeong-Il||Su, Shih-Chi

publication date

• January 2009

publisher

• Springer Nature  Publisher

published in

• Nature Protocols  Journal

keywords

• Angiogenesis Inducing Agents
• Animals
• Cell Culture Techniques
• Collagen
• Endothelial Cells
• Endothelium, Vascular
• Green Fluorescent Proteins
• Humans
• Neovascularization, Physiologic
• Rats

Digital Object Identifier (DOI)

• 10.1038/nprot.2009.221

start page

• 1888

end page

• 1898

volume

• 4

issue

• 12

URL

• http://dx.doi.org/10.1038/nprot.2009.221