Media composition influences yeast one- and two-hybrid results. Academic Article

abstract

• Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media preparation protocol and the source of the yeast nitrogen base may not only impact signal range but also effect whether a result appears positive or negative. While altering media preparation may optimize signal differences for individual experiments, media preparation must be reported in detail to replicate studies and accurately compare results from different experiments.

authors

• Bondos, Sarah

published proceedings

• Biol Proced Online

author list (cited authors)

• Liu, Y., Merchant, Z., Hsiao, H., Gonzalez, K. L., Matthews, K. S., & Bondos, S. E.

citation count

• 3

complete list of authors

• Liu, Ying||Merchant, Zabeena||Hsiao, Hao-Ching||Gonzalez, Kim L||Matthews, Kathleen S||Bondos, Sarah E

publication date

• January 2011

publisher

• Springer Nature  Publisher

published in

• Biological Procedures Online  Journal

keywords

• Accuracy
• False Negative
• False Positive
• Protein-protein Interaction
• Yeast One-hybrid
• Yeast Two-hybrid

Digital Object Identifier (DOI)

• 10.1186/1480-9222-13-6

URI

• https://hdl.handle.net/1969.1/180734

start page

• 6

volume

• 13

issue

• 1

URL

• http://dx.doi.org/10.1186/1480-9222-13-6