Transgenic zebrafish using transposable elements. Chapter

abstract

• DNA transposons are effective chromosomal engineering vehicles for making transgenic zebrafish. We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared within the zebrafish community. We present common transposon and transposase vectors within the field based on the Tol2 system. We describe methods with a high success rate of generating transgenic zebrafish using Tol2 vectors, including key quality control steps during the transgenesis process. Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry.

authors

• Clark, Karl

author list (cited authors)

• Clark, K. J., Urban, M. D., Skuster, K. J., & Ekker, S. C.

complete list of authors

• Clark, Karl J||Urban, Mark D||Skuster, Kimberly J||Ekker, Stephen C

Book Title

• The Zebrafish: Genetics, Genomics and Informatics

publication date

• January 2011

publisher

• Elsevier  Publisher

published in

• Methods in Cell Biology  Journal

keywords

• Animals
• Animals, Genetically Modified
• Cloning, Molecular
• DNA Transposable Elements
• Genetic Testing
• Genetic Vectors
• Germ-line Mutation
• Microinjections
• Mutagenesis, Insertional
• Zebrafish

PubMed Central ID

• 21924161

Digital Object Identifier (DOI)

• 10.1016/B978-0-12-374814-0.00008-2

start page

• 137

end page

• 149

volume

• 104

URL

• http://dx.doi.org/10.1016/b978-0-12-374814-0.00008-2