An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines. Academic Article

abstract

• The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed to be easily modified to work in diverse applications where editing of mitochondrial DNA is desired. For complete details on the use and execution of this protocol, please refer to Sabharwal etal. (2021) and Ma etal. (2016).

authors

• Clark, Karl

published proceedings

• STAR Protoc

author list (cited authors)

• Kar, B., Sabharwal, A., Restrepo-Castillo, S., Simone, B. W., Clark, K. J., & Ekker, S. C.

complete list of authors

• Kar, Bibekananda||Sabharwal, Ankit||Restrepo-Castillo, Santiago||Simone, Brandon W||Clark, Karl J||Ekker, Stephen C

publication date

• June 2022

publisher

• Elsevier  Publisher

published in

• STAR Protocols  Journal

keywords

• CRISPR
• Cell Biology
• Cell Line
• DNA, Mitochondrial
• Genetics
• Humans
• Mitochondria
• Molecular Biology
• Sequencing

PubMed Central ID

• 35496789

Digital Object Identifier (DOI)

• 10.1016/j.xpro.2022.101288

start page

• 101288

end page

• 101288

volume

• 3

issue

• 2

URL

• http://dx.doi.org/10.1016/j.xpro.2022.101288