The Azotobacter vinelandii nifL-like gene: nucleotide sequence analysis and regulation of expression. Academic Article

abstract

• The nucleotide sequence of the Azotobacter vinelandii nifL-like gene (Av-nifL) was determined. The 1.9 kb sequence shows an open reading frame (ORF) of 1577 bp which encodes a polypeptide of 519 amino acids, with a calculated molecular weight of 57,793. Av-nifL has about 50% homology with the Klebsiella pneumoniae nifL gene (Kp-nifL) at the nucleotide level and a little more than 52% homology at the amino acid level. The N-terminal regions show more homology than the C-terminal regions. As is the case in K. pneumoniae, Av-nifL is located just upstream of the A. vinelandii nifA gene (Av-nifA) and both genes constitute an operon. The expression of Av-nifL, however, seems to be independent of NtrA and NtrC. Furthermore, Av-nifL expression is not autogenously regulated by NifA, unlike the case in K. pneumoniae. The expression of an Av-nifL::lacZ fusion in A. vinelandii is inhibited by novobiocin and coumermycin A, which are inhibitors of DNA gyrase.

authors

• Bageshwar, Umesh

published proceedings

• Mol Gen Genet

author list (cited authors)

• Raina, R., Bageshwar, U. K., & Das, H. K.

citation count

• 20

complete list of authors

• Raina, R||Bageshwar, UK||Das, HK

publication date

• March 1993

publisher

• Springer Nature  Publisher

keywords

• Amino Acid Sequence
• Azotobacter Vinelandii
• Bacterial Proteins
• Base Sequence
• Cloning, Molecular
• DNA, Bacterial
• DNA, Superhelical
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Molecular Sequence Data
• Nitrogen Fixation
• Promoter Regions, Genetic
• Restriction Mapping
• Sequence Homology, Amino Acid

PubMed Central ID

• 8483455

Digital Object Identifier (DOI)

• 10.1007/BF00279444

start page

• 400

end page

• 406

volume

• 237

issue

• 3

URL

• http://dx.doi.org/10.1007/bf00279444