Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway inAzotobacter vinelandii
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A lac fusion of the vnfH promoter of Azotobacter vinelandii has been constructed. An upstream sequence seems to be necessary for the activity of the promoter. The repression of expression of this promoter by fixed nitrogen is several-fold lower compared to that of the nifH promoter. Vanadium has no role in the expression of the nifH or the vnfH promoter. Molybdenum is essential for the nifH promoter and represses the vnfH promoter. Tungsten can substitute molybdenum in its regulatory role. ntrA and vnfA are essential for the expression of vnfH, but ntrC has no role. 1992.
FEMS Microbiology Letters
author list (cited authors)
Raina, R., Bageshwar, U. K., & Das, H. K.
complete list of authors
Raina, Ramesh||Bageshwar, Umesh K||Das, HK