The TolC and Lipopolysaccharide-Specific Escherichia coli Bacteriophage TLS-the Tlsvirus Archetype Virus.

abstract

• INTRODUCTION: TLS is a virulent bacteriophage of Escherichia coli that utilizes TolC and lipopolysaccharide as its cell surface receptors. METHODS: The genome was reannotated using the latest online resources and compared to other T1-like phages. RESULTS: The TLS genome consists of 49,902 base pairs, encoding 86 coding sequences that display considerable sequence similarity with the T1 phage genome. It also contains 18 intergenic 21-base long repeats, each of them upstream of a predicted start codon and in the direction of transcription. Data revealed that DNA packaging occurs through the pac site-mediated headful mechanism. CONCLUSIONS: Based on sequence analysis of its genome, TLS belongs to the Drexlerviridae family and represents the type member of the Tlsvirus genus.

authors

• Ramsey, Jolene

published proceedings

• Phage (New Rochelle)

author list (cited authors)

• German, G. J., DeGiulio, J. V., Ramsey, J., Kropinski, A. M., & Misra, R.

complete list of authors

• German, Gregory J||DeGiulio, James V||Ramsey, Jolene||Kropinski, Andrew M||Misra, Rajeev

publisher

• Mary Ann Liebert  Publisher

published in

• PHAGE: Therapy, Applications, and Research  Journal

keywords

• Bacteriophages
• Drexlerviridae
• Genomics
• Host-receptors
• Proteome
• Tempevirinae

PubMed Central ID

• 39372356

Digital Object Identifier (DOI)

• 10.1089/phage.2023.0041

start page

• 173

end page

• 183

volume

• 5

issue

• 3