Real-time analysis of rhythmic gene expression in immortalized suprachiasmatic nucleus cells. Academic Article

abstract

• Immortalized cells derived from the suprachiasmatic nucleus (SCN) retain many properties of the SCN including the capacity to generate circadian rhythms. Stably transfected SCN2.2 cells expressing the human c- promoter linked to a luciferase reporter gene ( /luc) were examined for evidence of transgene responses to stimuli known to induce c- expression and of endogenous rhythmic variation. Bioluminescence-reported transgene expression was induced in SCN2.2 /luc cells following stimulation with fetal bovine serum or KCl. SCN2.2 /luc cells showed 24 h rhythms of bioluminescence with a 9- to 19-fold difference between peak and minimum levels. These results demonstrate that the regulation of /luc transgene expression in SCN2.2 cells is similar to that of the endogenous c- gene in the SCN.

authors

• Allen, Gregg
• Earnest, David

published proceedings

• Neuroreport

author list (cited authors)

• Allen, G. C., & Earnest, D. J.

citation count

• 4

complete list of authors

• Allen, Gregg C||Earnest, David J

publication date

• January 2002

publisher

• Wolters Kluwer  Publisher

published in

• NeuroReport  Journal

keywords

• Animals
• Cell Line
• Circadian Rhythm
• Gene Expression
• Genes, Fos
• Luciferases
• Rats
• Suprachiasmatic Nucleus
• Transfection

PubMed Central ID

• 12438919

Digital Object Identifier (DOI)

• 10.1097/00001756-200211150-00007

start page

• 2027

end page

• 2030

volume

• 13

issue

• 16

URL

• http://dx.doi.org/10.1097/00001756-200211150-00007