Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy. Academic Article uri icon

abstract

  • This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (10(5) cells/well) for 24 h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1 g mL(-1)), followed by LLLT irradiation (LaserTABLE-InGaAsP diode prototype-780 nm, 25 mW) delivering 0, 0.5, 1.5 or 3 J cm(-2). Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-, IL-1, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P < 0.05). LPS treatment increased the gene expression and protein synthesis of TNF-, IL-6 and IL-8, while the expression of IL-1 was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-, IL-6, and IL-8 at 1.5 J cm(-2) and 3 J cm(-2). These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of inflammatory cytokines related to oral mucositis by human gingival fibroblasts.

published proceedings

  • Photochem Photobiol

author list (cited authors)

  • Basso, F. G., Pansani, T. N., Soares, D. G., Scheffel, D. L., Bagnato, V. S., de Souza Costa, C. A., & Hebling, J.

complete list of authors

  • Basso, Fernanda G||Pansani, Taisa N||Soares, Diana G||Scheffel, Débora L||Bagnato, Vanderlei S||de Souza Costa, Carlos Alberto||Hebling, Josimeri

publication date

  • July 2015

publisher