Photodynamic Inactivation of Cariogenic Pathogens Using Curcumin as Photosensitizer. Academic Article uri icon

abstract

  • OBJECTIVE: This investigation assessed the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to Photodynamic Therapy (PDT) when grown simultaneously in dentine carious lesions. BACKGROUND DATA: PDT is a technique that utilizes light to activate photosensitizers in the presence of oxygen to produce reactive radicals. MATERIALS AND METHODS: A culture medium of 1% glucose, 2% sucrose, 1% young primary culture of L. acidophilus 108 CFU/mL, and S. mutans 108 CFU/mL was utilized to inoculate the bacterial induced caries on human dentine slabs. Different concentrations of the photosensitizer (0.75, 1.5, 3.0, 4.0, and 5.0g/L) were activated through exposure to the light-emitting diode source with a central wavelength of 450nm and a fluency of 5.7J/cm2. Two light intensities (19 and 47.5mW/cm2) were tested. Four different groups were analyzed: L-D- (control group), L-D+ (drug group), L+D+1 (PDT group 1, light intensity of 19mW/cm2), and L+D+2 (PDT group 2, light intensity of 47.5mW/cm2). ANOVA/Tukey tests were utilized to compare groups (=5%). RESULTS: Both light intensities required 5.0g/L of curcumin for significant bacterial reduction (p<0.05). No significant effect was found for L-D+, thus proving the absence of a potential inherent toxicity. CONCLUSIONS: Curcumin has a toxic effect on microorganisms at appreciable concentrations upon photoactivation. However, it was required to use the maximum concentration of the drug for a successful procedure.

published proceedings

  • Photomed Laser Surg

author list (cited authors)

  • Arajo, N. C., de Menezes, R. F., Carneiro, V., Dos Santos-Neto, A. P., Fontana, C. R., Bagnato, V. S., Harvey, C. M., & Gerbi, M.

complete list of authors

  • Araújo, Natália Costa||de Menezes, Rebeca Ferraz||Carneiro, Vanda Sanderana Macêdo||Dos Santos-Neto, Alexandrino Pereira||Fontana, Carla Raquel||Bagnato, Vanderlei Salvador||Harvey, Catherine Malinda||Gerbi, Marleny Elizabeth Martinez

publication date

  • May 2017