Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination Using Cryo-Electron Microscopy. Chapter

abstract

• Single-stranded RNA bacteriophages (ssRNA phages) are small viruses with a compact genome (~3-4kb) that infect gram-negative bacteria via retractile pili. These phages have been applied in various fields since their discovery approximately 60years ago. To understand their biology, it is crucial to analyze the structure of mature virions. Cryo-electron microscopy (cryo-EM) has been employed to determine the structures of two ssRNA phages, MS2 and Q. This chapter presents a method for purifying these two phages and their receptor, the F-pilus, to allow examination using cryo-EM.

authors

• Zhang, Junjie

author list (cited authors)

• Thongchol, J., & Zhang, J.

complete list of authors

• Thongchol, Jirapat||Zhang, Junjie

Book Title

• Phage Engineering and Analysis

publication date

• January 2024

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Bacteriophages
• Cryo-em
• Cryoelectron Microscopy
• F-pilus
• Fimbriae, Bacterial
• Levivirus
• Ms2
• Qβ
• RNA, Viral
• Ssrna Phages

PubMed Central ID

• 38526732

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-3798-2_13

International Standard Book Number (ISBN) 13

• 9781071637975

start page

• 185

end page

• 204

volume

• 2793

URL

• http://dx.doi.org/10.1007/978-1-0716-3798-2_13