Evaluation of a novel Tc-24 recombinant antigen ELISA for serologic testing for Trypanosoma cruzi in dogs. uri icon

abstract

  • Chagas disease is a parasitic infection caused by Trypanosoma cruzi. Diagnosis of chronic Chagas disease in dogs relies on limited serological test options. This study used a new Tc-24 recombinant antigen ELISA on an archival set of 70 dog serum samples from multi-dog kennel environments in Texas subjected to three existing Chagas serological tests. Tc-24 ELISA produced a quantitative result and could detect anti-T. cruzi antibodies in dogs with high sensitivity and specificity. Comparing individual tests to Tc-24 ELISA resulted in strong associations and correlations, which suggest that Tc-24 ELISA is a reliable and accurate diagnostic tool for dogs with a single test.

published proceedings

  • bioRxiv

author list (cited authors)

  • Mejia, R., Verocai, G. G., Mosley, I. A., Zhan, B., Vongthavaravat, L., Busselman, R. E., & Hamer, S. A.

complete list of authors

  • Mejia, Rojelio||Verocai, Guilherme G||Mosley, Ilana A||Zhan, Bin||Vongthavaravat, Lindsey||Busselman, Rachel E||Hamer, Sarah A