Partite expression of the bovine papillomavirus E1 open reading frame in Escherichia coli.

abstract

• Six recombinants were constructed which expressed portions of the bovine papillomavirus E1 open reading frame as OmpF/E1/beta-galactosidase tribrid fusion proteins in Escherichia coli. Rabbit sera containing E1-specific antibodies were generated against five of these six fusion proteins (which together constitute 74% of the full-length E1 open reading frame). The individual fusion proteins and their cognate antisera will be useful reagents for defining the structure and function of the BPV E1 protein(s).

authors

• Wilson, Van

published proceedings

• Biochim Biophys Acta

author list (cited authors)

• Wilson, V. G., & Ludes-Meyers, J.

citation count

• 8

complete list of authors

• Wilson, VG||Ludes-Meyers, J

publication date

• January 1992

publisher

• Elsevier  Publisher

published in

• Biochimica et Biophysica Acta: international journal of biochemistry and biophysics  Journal

keywords

• Bacterial Outer Membrane Proteins
• Beta-Galactosidase
• Bovine Papillomavirus 1
• DNA-Binding Proteins
• Escherichia Coli
• Immune Sera
• Open Reading Frames
• Precipitin Tests
• Recombinant Fusion Proteins
• Viral Proteins

Digital Object Identifier (DOI)

• 10.1016/0167-4781(92)90490-q

start page

• 215

end page

• 218

volume

• 1129

issue

• 2

URL

• http://dx.doi.org/10.1016/0167-4781(92)90490-q