Changes in DNA binding by purified simian RNA polymerase II under transcribing and nontranscribing conditions. Academic Article uri icon


  • The interaction of RNA polymerase II from African Green Monkey liver tissue with SV40 DNA was examined by a DNAase protection technique. In the absence of nucleoside triphosphates, simian polymerase binds to nicked, linear SV40 DNA and protects 30 bp of binary complex DNA from DNAase I digestion. With the addition of nucleoside triphosphates to initiate transcription, polymerase protects 40 bp of the ternary complex DNA from DNAase I. Thus, a conformational change in either the polymerase, the DNA, or both occurs during the transition from binary to ternary complex, and this altered conformation allows a larger protection of template DNA. Similar results were seen with Escherichia coli RNA polymerase holoenzyme on SV40 DNA.

published proceedings

  • Biochim Biophys Acta

author list (cited authors)

  • Wilson, V. G., & Spillman, T.

citation count

  • 0

complete list of authors

  • Wilson, VG||Spillman, T

publication date

  • December 1982