Growth and differentiation of HaCaT keratinocytes. Chapter uri icon

abstract

  • HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent controlled differentiation using calcium induction.

author list (cited authors)

  • Wilson, V. G.

citation count

  • 56

complete list of authors

  • Wilson, Van G

Book Title

  • EPIDERMAL CELLS: METHODS AND PROTOCOLS, 3RD EDITION

publication date

  • January 2014