Rapid Waterborne Pathogen Detection with Mobile Electronics. Academic Article uri icon


  • Pathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal-oxide-semiconductor (CMOS) imagers from mobile electronics, a low-cost and one-step reaction detection protocol is developed to enable field detection for waterborne pathogens. 10 mL of pathogen-containing water samples was processed using the developed protocol including filtration enrichment, immune-reaction detection and imaging processing. The limit of detection of 10 E. coli O157:H7 cells/10 mL has been demonstrated within 10 min of turnaround time. The protocol can readily be integrated into a mobile electronics such as smartphones for rapid and reproducible field detection of waterborne pathogens.

published proceedings

  • Sensors (Basel)

author list (cited authors)

  • Wu, T., Chen, Y., Wang, W., Kucknoor, A. S., Lin, C., Lo, Y., Yao, C., & Lian, I.

citation count

  • 11

complete list of authors

  • Wu, Tsung-Feng||Chen, Yu-Chen||Wang, Wei-Chung||Kucknoor, Ashwini S||Lin, Che-Jen||Lo, Yu-Hwa||Yao, Chun-Wei||Lian, Ian

publication date

  • June 2017