Detection of Salmonella enteritidis in Feces from Poultry Using Booster Polymerase Chain Reaction and Oligonucleotide Primers Specific for All Members of the Genus Salmonella Academic Article uri icon

abstract

  • Salmonella enteritidis was identified in feces from hens using the polymerase chain reaction (PCR) and oligonucleotide primers specific for all members of the genus Salmonella. Feces from specific-pathogen-free Leghorn hens were determined to be negative for Salmonella by microbiological culture and by the PCR. Fecal samples were inoculated with known numbers of colony-forming units of S. enteritidis. The DNA was extracted from fecal samples and amplified by the PCR using genus-specific primers. Salmonella were detected in all samples known to be positive; the sensitivity of the assay extended to 1 cfu of S. enteritidis/g feces. Feces that were not inoculated with Salmonella were negative. Microbiological culture was less sensitive than the PCR assay; results of culture of feces with less than 10(2) cfu/g were negative. Although S. enteritidis was used in this study, the oligonucleotide primers used in this study have been previously demonstrated to be genus-specific for Salmonella.

author list (cited authors)

  • COHEN, N. D., MCGRUDER, E. D., NEIBERGS, H. L., BEHLE, R. W., WALLIS, D. E., & HARGIS, B. M.

citation count

  • 29

publication date

  • February 1994