Detection of Cryptosporidium parvum in horses: thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry. Academic Article

abstract

• Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for the IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.

authors

• Cohen, Noah
• Smith, Roger
• Snowden, Karen

published proceedings

• J Clin Microbiol

author list (cited authors)

• Cole, D. J., Snowden, K., Cohen, N. D., & Smith, R.

citation count

• 22

complete list of authors

• Cole, DJ||Snowden, K||Cohen, ND||Smith, R

publication date

• February 1999

publisher

• American Society for Microbiology  Publisher

keywords

• Animals
• Cryptosporidiosis
• Cryptosporidium Parvum
• Feces
• Flow Cytometry
• Fluorescent Antibody Technique
• Horse Diseases
• Horses
• Sensitivity And Specificity
• Staining And Labeling

Digital Object Identifier (DOI)

• 10.1128/JCM.37.2.457-460.1999

URI

• https://hdl.handle.net/1969.1/180343

start page

• 457

end page

• 460

volume

• 37

issue

• 2

URL

• http://dx.doi.org/10.1128/jcm.37.2.457-460.1999