Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)
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Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.
author list (cited authors)
Cohen, N. D., Neibergs, H. L., McGruder, E. D., Whitford, H. W., Behle, R. W., Ray, P. M., & Hargis, B. M.