Genus-specific detection of salmonellae using the polymerase chain reaction (PCR).
Academic Article
Overview
Research
Identity
Additional Document Info
Other
View All
Overview
abstract
Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.