Methods for Engineering Binders to Multi-Pass Membrane Proteins Academic Article

abstract

• Numerous potential drug targets, including G-protein-coupled receptors and ion channel proteins, reside on the cell surface as multi-pass membrane proteins. Unfortunately, despite advances in engineering technologies, engineering biologics against multi-pass membrane proteins remains a formidable task. In this review, we focus on the different methods used to prepare/present multi-pass transmembrane proteins for engineering target-specific biologics such as antibodies, nanobodies and synthetic scaffold proteins. The engineered biologics exhibit high specificity and affinity, and have broad applications as therapeutics, probes for cell staining and chaperones for promoting protein crystallization. We primarily cover publications on this topic from the past 10 years, with a focus on the different formats of multi-pass transmembrane proteins. Finally, the remaining challenges facing this field and new technologies developed to overcome a number of obstacles are discussed.

authors

• Chen, Zhilei

published proceedings

• Bioengineering

altmetric score

• 0.5

author list (cited authors)

• Thomas, B., Chockalingam, K., & Chen, Z.

citation count

• 0

complete list of authors

• Thomas, Benjamin||Chockalingam, Karuppiah||Chen, Zhilei

publisher

• MDPI  Publisher

published in

• Bioengineering  Journal

keywords

• 4 Quality Education
• 40 Engineering
• 4003 Biomedical Engineering
• Bioengineering
• Biotechnology
• Generic Health Relevance
• Prevention

Digital Object Identifier (DOI)

• 10.3390/bioengineering10121351

start page

• 1351

end page

• 1351

volume

• 10

issue

• 12

URL

• http://dx.doi.org/10.3390/bioengineering10121351