Jasmonic Acid (JA) is one of the important substances that associates with stomatal closure under drought stress for maize. In this project, we performed multiple network analyses to identify the expression patterns of JA pathway genes in drought responses of different maize genotypes where certain genes from the related pathway were knocked out. Differential gene expression analysis was performed to identify differentially expressed genes across different water conditions for each maize genotype, respectively. As the number of samples is limited in this project, we used partial correlation for co-expression network construction for different genotypes using the identified DEGs and a weighted LASSO regression method that applies to all samples for detailed network connectivity analyses of the JA pathway. The results reveal the changes of behaviors and regulatory relationships of JA pathway genes in different genotypes. We discussed the shifting of behaviors between different genotypes from the analysis results.
Jasmonic Acid (JA) is one of the important substances that associates with stomatal closure under drought stress for maize. In this project, we performed multiple network analyses to identify the expression patterns of JA pathway genes in drought responses of different maize genotypes where certain genes from the related pathway were knocked out. Differential gene expression analysis was performed to identify differentially expressed genes across different water conditions for each maize genotype, respectively. As the number of samples is limited in this project, we used partial correlation for co-expression network construction for different genotypes using the identified DEGs and a weighted LASSO regression method that applies to all samples for detailed network connectivity analyses of the JA pathway. The results reveal the changes of behaviors and regulatory relationships of JA pathway genes in different genotypes. We discussed the shifting of behaviors between different genotypes from the analysis results.
