The Effect of Acute Highintensity Resistance Exercise and Dietary Cholesterol on PPAR Protein Content in Skeletal Muscle Academic Article uri icon


  • PPAR expression in skeletal muscle has been shown to increase in response to endurance exercise training. However, the effect of acute highintensity resistance exercise (RE) and/or dietary cholesterol (CL) intake on PPAR expression has not been investigated in humans and is thus the purpose of this study. Fourteen untrained, healthy young men and women were randomly assigned to a highcholesterol (HC: 14mg/kg lean/day, n=8) or lowcholesterol intake group (LC: 3.5mg/kg lean/day, n=6), and instructed to perform 2 bouts of eccentric RE consisting of unilateral leg presses and extensions to fatigue. Muscle biopsies were performed on both exercise (EX) and nonexercise (NoEX) legs 22 hours after exercise. PPAR levels were analyzed via Western Blotting. The results showed no significant difference in PPAR levels between HC and LC groups (p=0.868) or EX and NoEX legs (p=0.325). However, there was a significant interaction between RE and CL intake in PPAR levels (p=0.047). In HC group, PPAR levels in EX legs (1.810.44 AU) were higher than NoEX legs (1.040.15 AU) by 74% (p=0.029) while exercise had no effect on PPAR levels in LC group (EX: 1.360.31 AU, NoEX: 1.640.44 AU, p=0.445). Our data suggest that when combined with high CL intake, acute highintensity RE may enhance PPAR expression while acute highintensity RE or dietary CL alone has no effect on PPAR levels.

published proceedings

  • The FASEB Journal

author list (cited authors)

  • Chen, V., Lee, C. W., Lee, T. V., Bui, S., & Riechman, S. E.

complete list of authors

  • Chen, Vincent CW||Lee, Chang Woock||Lee, Teak V||Bui, Steve||Riechman, Steven E

publication date

  • April 2011