HUMAN BREAST-CANCER CELL-LINES AS MODELS FOR INVESTIGATING THE EFFECTS OF 2,3,7,8-TCDD AND RELATED-COMPOUNDS Conference Paper

abstract

• The dose-response effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF) and 1,2,4,7,8-pentachlorodibenzo-p-dioxin (PeCDD) as inducers of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD) were determined for 3 human breast cancer cell lines. The T47-D and MCF-7 cell lines were highly responsive to the induction effects of TCDD and TCDF. EC50 values were approximately 1 10-9 M for TCDD and TCDF in both cell lines and in both enzyme assays. These results, coupled with the low activity of the PeCDD congener were consistent with an Ah receptor mediated-induction process. The MDA-MB-231 cell line was not responsive to TCDD, TCDF or PeCDD. Preliminary data demonstrated that pretreatment of MCF-7 cells with TCDD resulted in a substantial decrease in the amount of nuclear estrogen receptor bound by [3H]-estradiol. Additional studies using estrogen receptor antibodies confirmed the reduction of the nuclear estrogen receptor protein in MCF-7 cells by TCDD. 1990.

authors

• Safe, Stephen

published proceedings

• CHEMOSPHERE

author list (cited authors)

• HARRIS, M., PISKORSKAPLISZCZYNSKA, J., ZACHAREWSKI, T., & SAFE, S.

citation count

• 0

complete list of authors

• HARRIS, M||PISKORSKAPLISZCZYNSKA, J||ZACHAREWSKI, T||SAFE, S

publication date

• January 1990

publisher

• Elsevier  Publisher

published in

• Chemosphere  Journal

keywords

• 31 Biological Sciences
• 3101 Biochemistry And Cell Biology
• 32 Biomedical And Clinical Sciences
• 3211 Oncology And Carcinogenesis
• Agent Orange & Dioxin
• Breast Cancer
• Cancer
• Estrogen

Digital Object Identifier (DOI)

• 10.1016/0045-6535(90)90233-J

start page

• 1135

end page

• 1140

volume

• 20

issue

• 7-9

URL

• http://dx.doi.org/10.1016/0045-6535(90)90233-j