The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation. Academic Article

abstract

• A comparison was made of various indices of the hepatic drug-metabolizing apparatus associated with the postmitochondrial superntant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone-pretreated and 3-methylcholanthrene-pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P-450 and by the activity of NADPH-cytochrome c reductase, 4-dimethylaminoantipyrine N-demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes.

authors

• Safe, Stephen

published proceedings

• J Pharm Pharmacol

author list (cited authors)

• Parkinson, A., & Safe, S.

citation count

• 31

complete list of authors

• Parkinson, A||Safe, S

publication date

• July 1979

publisher

• Oxford University Press (OUP)  Publisher

published in

• Journal of Pharmacy and Pharmacology  Journal

keywords

• Animals
• Benzopyrene Hydroxylase
• Centrifugation, Density Gradient
• Cytochrome P-450 Enzyme System
• Cytochromes
• Enzyme Induction
• Isoelectric Point
• Liver
• Male
• Methylcholanthrene
• Microsomes, Liver
• Nadph-ferrihemoprotein Reductase
• Oxidoreductases, N-Demethylating
• Phenobarbital
• Rats

PubMed Central ID

• 38312

Digital Object Identifier (DOI)

• 10.1111/j.2042-7158.1979.tb13550.x

start page

• 444

end page

• 447

volume

• 31

issue

• 7

URL

• http://dx.doi.org/10.1111/j.2042-7158.1979.tb13550.x