Kinetics of myogenic progenitor cell (MPC) expansion and bone marrowderived cell recruitment in skeletal muscle regeneration Conference Paper uri icon

abstract

  • Muscle regeneration requires myogenic progenitor cells (MPC) and recruitment of bone marrow (BM)derived cells. MPC (CD34+/CD45/Sca1) and BMderived progenitor cells (CD45+/Sca1+) isolated from injured muscle undergo myogenic differentiation in vitro. However, the kinetics of MPC expansion and BMderived cell recruitment to injured muscle are poorly understood and were quantitated in tibialis anterior muscle using flow cytometry after cardiotoxin (CTX) injury in wild type (WT, C57Bl/6J) male mice. Maximal MPC expansion occurred 3 days postCTX and BMderived progenitor and immune cell recruitment exhibited similar kinetics; few cells present at baseline with maximal accumulation 3 days postCTX.MPC expansion and BMderived cell recruitment occurred within days of injury and demonstrated similar accumulation kinetics suggesting interactions between MPC and BMderived cells may be crucial to skeletal muscle regeneration. A better understanding of interactions between different cell types could result in improved strategies for muscle regeneration and tissue engineering.Supported by Veterans Administration and HL074236 and HL090196.

published proceedings

  • The FASEB Journal

author list (cited authors)

  • Martinez, C. O., McManus, L. M., & Shireman, P. K.

citation count

  • 0

complete list of authors

  • Martinez, Carlo Obet||McManus, Linda M||Shireman, Paula K

publication date

  • April 2009

publisher