MiRNA Expression Patterns during Skeletal Muscle Regeneration Conference Paper uri icon


  • MiRNAs regulate many biological processes. Expression of miRNAs during mouse muscle (tibialis anterior) regeneration was analyzed by qPCRarray at baseline and days 1, 3, 4, 7, 21 postcardiotoxin injury. Based on histology, satellite cell proliferation occurred at days 14 after injury and myoblast differentiation began at day 3, with overlap of both events at days 34. Over 100 miRNAs changed >2fold (p<0.01) after injury compared to baseline. miRNA Fold Change Day 1 3 4 7 21 miR1 104 94 28 4.6 1.6 miR133a 32 127 34 5.2 1.7 miR682 7.4 5.2 1.5 2.8 1.8 Expression of musclespecific miR1 and 133a significantly decreased immediately after injury and returned to baseline, a pattern consistent with a role in maintaining muscle differentiation. Conversely, another subset of miRNAs, including miR682, significantly increased at early days after injury, suggesting regulatory roles in satellite cell proliferation. In addition, some miRNAs were upregulated in both early and late days after injury. Interestingly, the expression time course of the above 3 miRNAs was remarkably similar during in vivo muscle regeneration and in vitro myogenic progenitor cell proliferation and differentiation. These studies define miRNA expression patterns that may regulate muscle repair; manipulation of specific miRNAs may improve muscle regeneration. Supported by the Veterans Administration HL07446 and HL074236.

published proceedings

  • The FASEB Journal

author list (cited authors)

  • Chen, Y., Gelfond, J., McManus, L. M., & Shireman, P. K.

citation count

  • 0

complete list of authors

  • Chen, Yongxin||Gelfond, Jonathan||McManus, Linda M||Shireman, Paula K

publication date

  • April 2010