Unique microRNA expression patterns associated with macrophage infiltration following skeletal muscle injury Conference Paper uri icon


  • Skeletal muscle regeneration following injury requires macrophages. MicroRNAs (miRNAs) are vital to macrophage biology and may be key regulators of muscle regeneration. We compared temporal expression of miRNA in cardiotoxininjured muscle derived from wild type (WT, C57Bl/6J) and CCR2/ mice to identify macrophagespecific miRNA patterns. MiRNA expression was determined on muscle RNA by qRTPCR in TaqMan arrays. Compared to uninjured muscle, logfold changes in miRNA expression at 1, 3, or 7 days following injury (n=4 mice/group/timepoint) were determined. Flow cytometry confirmed impaired macrophage recruitment in CCR2/ mice. The temporal expression patterns of 60 miRNAs were significantly different between strains, including miRNAs known to be highly expressed in macrophages (e.g., miR146a, miR106b, and miR221). Interestingly, 15 miRNAs with no known relationship to macrophages were matched with the temporal expression patterns of macrophagerelated miRNAs. These 15 miRNAs may be unique, macrophagerelated miRNA that regulate the muscle regenerative response. This study furthers our knowledge of miRNA regulation of the regenerative response in injured skeletal muscle and identifies potential miRNA targets for advancing tissue regeneration following traumatic injury. Funding: Veterans Administration and NIH (HL110743 and HL074236).

published proceedings

  • The FASEB Journal

author list (cited authors)

  • Melton, D. W., Gelfond, J., Chen, Y., McManus, L., & Shireman, P.

citation count

  • 0

complete list of authors

  • Melton, David Wayne||Gelfond, Jonathan||Chen, Yongxin||McManus, Linda||Shireman, Paula

publication date

  • April 2012