Aminophospholipid asymmetry in murine synaptosomal plasma membrane. Academic Article uri icon


  • The asymmetric distribution of aminophospholipids in isolated murine synaptosomal plasma membranes was determined by a chemical labeling procedure. Under nonpenetrating conditions, mouse brain synaptosomes were reacted with trinitrobenzenesulfonic acid (TNBS) to label outermonolayer aminophospholipids covalently. About 1015% of the phosphatidylethanolamine and 20% of the phosphatidylserine were found to be in the outer monolayer of the synaptosomal plasma membrane. Furthermore, the fatty acyl group composition of the labeled phosphatidylethanolamine (outer monolayer) consisted of more saturated fatty acid than did the unlabeled phosphatidylethanolamine (inner monolayer). These results demonstrated an aminophospholipid asymmetry in synaptosomal plasma membranes which was independent of serumlipoprotein exchange processes and also of phosphatidylethanolaminemethylatingenzymes. Copyright 1980, Wiley Blackwell. All rights reserved

published proceedings

  • J Neurochem

author list (cited authors)

  • Fontaine, R. N., Harris, R. A., & Schroeder, F.

citation count

  • 117

complete list of authors

  • Fontaine, RN||Harris, RA||Schroeder, F

publication date

  • February 1980