Chromosome-level reference genome for North American bison (Bison bison) and variant database aids in identifying albino mutation.
Academic Article
Overview
Research
Identity
Additional Document Info
Other
View All
Overview
abstract
We developed a highly contiguous chromosome-level reference genome for North American bison to provide a platform to evaluate the conservation, ecological, evolutionary, and population genomics of this species. Generated from a F1 hybrid between a North American bison dam and a domestic cattle bull, completeness and contiguity exceed that of other published bison genome assemblies. To demonstrate the utility for genome-wide variant frequency estimation, we compiled a genomic variant database consisting of 3 true albino bison and 44 wild-type pelage color bison. Through the examination of genomic variants fixed in the albino cohort and absent in the controls, we identified a nonsynonymous single nucleotide polymorphism (SNP) mutation on chromosome 29 in exon 3 of the tyrosinase gene (c.1114C>T). A TaqMan SNP Genotyping Assay was developed to genotype this SNP in a total of 283 animals across 29 herds. This assay confirmed the absence of homozygous variants in all animals except 7 true albino bison included in this study. In addition, the only heterozygous animals identified were 2 wild-type pelage color dams of albino offspring. Therefore, we propose that this new high-quality bison genome assembly and incipient variant database provides a highly robust and informative resource for genomics investigations for this iconic North American species.
Stroupe, S., Martone, C., McCann, B., Juras, R., Kjllerstrm, H. J., Raudsepp, T., ... Derr, J. N.
complete list of authors
Stroupe, Sam||Martone, Carly||McCann, Blake||Juras, Rytis||Kjöllerström, Helena Josefina||Raudsepp, Terje||Beard, Donald||Davis, Brian W||Derr, James N
