Uveitis, vitreous humor, and klebsiella. I. Binding studies with rabbit antisera. Academic Article

abstract

• Uveitis occur in a proportion of patients with ankylosing spondylitis, and an increased faecal isolation of the Gram-negative micro-organism Klebsiella pneumoniae has been reported from such patients. Immunological cross-reactivity between K. pneumoniae and bovine vitreous humour has been studied by 2 different antibody binding techniques: I125-labelled antigen binding assay with and without carrier, and beta-galactosidase enzyme-immunoassay. Sera from rabbits immunised with whole klebsiella micro-organisms or klebsiella extracts were found to bind labelled vitreous humour antigens to a greater extent (p less than 0.001) than sera from rabbits immunised with Escherichia coli, Streptococcus pyogenes, and phi X 174 virus or sera from the same rabbits before immunisation. It is suggested klebsiella micro-organisms may carry antigenic determinants which resemble vitreous humour antigens.

authors

• Welsh, Christabel Jane

published proceedings

• Br J Ophthalmol

author list (cited authors)

• Avakian, H., Abuknesha, R., Welsh, J., & Ebringer, A.

citation count

• 16

complete list of authors

• Avakian, H||Abuknesha, R||Welsh, J||Ebringer, A

publication date

• May 1981

publisher

• BMJ  Publisher

published in

• British Journal of Ophthalmology  Journal

keywords

• Animals
• Binding Sites, Antibody
• Cattle
• Cross Reactions
• Epitopes
• Humans
• Immune Sera
• Immunoenzyme Techniques
• Klebsiella Pneumoniae
• Rabbits
• Radioligand Assay
• Uveitis, Anterior
• Vitreous Body

PubMed Central ID

• 6166317

Digital Object Identifier (DOI)

• 10.1136/bjo.65.5.315

start page

• 315

end page

• 322

volume

• 65

issue

• 5

URL

• http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981LR60200004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=dd60ee4408902e709853f976e23bc0ff