A new DNA marker, U15557, is linked to protease inhibitor and adenylate kinase-1 in the laboratory opossum, Monodelphis domestica. Academic Article

abstract

• A 323-bp DNA fragment (U15557) was isolated, cloned, and sequenced after polymerase chain reaction (PCR) amplification from Monodelphis domestica genomic DNA. A HindIII restriction fragment length polymorphism was identified in this species using the U15557 PCR, fragment as a hybridization probe. DNA samples exhibited either a 6.4 kb band, a 7.2 kb band, or both bands simultaneously. Behaviour of these two variants in family studies was consistent with codominant autosomal inheritance. Linkage between this marker and the loci encoding protease inhibitor (PI) and adenylate kinase 1 (AK1) was found in M. domestica.

authors

• Samollow, Paul

published proceedings

• Anim Genet

author list (cited authors)

• Perelygin, A. A., Samollow, P. B., Perelygina, L. M., Cherry, L. M., Mahaney, S. M., & VandeBerg, J. L.

citation count

• 5

complete list of authors

• Perelygin, AA||Samollow, PB||Perelygina, LM||Cherry, LM||Mahaney, SM||VandeBerg, JL

publication date

• April 1996

publisher

• Wiley  Publisher

published in

• Animal Genetics  Journal

keywords

• Adenylate Kinase
• Animals
• Cloning, Molecular
• Crosses, Genetic
• Female
• Genetic Linkage
• Genetic Markers
• Male
• Molecular Sequence Data
• Opossums
• Polymorphism, Restriction Fragment Length
• Protease Inhibitors
• Sequence Analysis, DNA
• Sex Factors

PubMed Central ID

• 8856903

Digital Object Identifier (DOI)

• 10.1111/j.1365-2052.1996.tb00479.x

start page

• 113

end page

• 116

volume

• 27

issue

• 2

URL

• http://dx.doi.org/10.1111/j.1365-2052.1996.tb00479.x