In vitro and in silico annotation of conserved and nonconserved microRNAs in the genome of the marsupial Monodelphis domestica. Academic Article

abstract

• The gray short-tailed opossum, Monodelphis domestica, is the world's most widely utilized marsupial model for biomedical research. Recent completion of the initial M. domestica genome assembly offers the first opportunity to examine genome-wide phenomena in a marsupial. Using in silico methods, we have mapped 124 conserved microRNAs (miRNAs) to 94 loci in the M. domestica genome. In addition, using RNA pooled from 5 tissues, we cloned 85 miRNAs. Seventy-two of these are conserved miRNAs that we had mapped in silico. The additional 13 are nonconserved candidate miRNAs in 11 loci. Nine of these 13 are also found in the wallaby (Macropus eugenii) genome. Two of the candidate miRNA clones, located on the X chromosome, are part of a cluster containing a total of 24 potential miRNAs spanning more than 100 kb.

authors

• Samollow, Paul

published proceedings

• J Hered

altmetric score

• 3

author list (cited authors)

• Devor, E. J., & Samollow, P. B.

citation count

• 10

complete list of authors

• Devor, Eric J||Samollow, Paul B

publication date

• January 2008

publisher

• Oxford University Press (OUP)  Publisher

published in

• JOURNAL OF HEREDITY  Journal

keywords

• Animals
• Base Sequence
• Chromosome Mapping
• Computational Biology
• Conserved Sequence
• Genome
• Humans
• Macropodidae
• Mice
• MicroRNAs
• Molecular Sequence Data
• Monodelphis
• Sequence Analysis, RNA

PubMed Central ID

• 17965199

Digital Object Identifier (DOI)

• 10.1093/jhered/esm085

start page

• 66

end page

• 72

volume

• 99

issue

• 1

URL

• http://dx.doi.org/10.1093/jhered/esm085