The effect of chlortetracycline on faecal microbial populations in growing swine. Academic Article uri icon

abstract

  • The effect of antimicrobial use on the gastrointestinal microbiota of food animals is of increasing concern as bacteria accumulate resistance to multiple antimicrobials. Only a small fraction of the gastrointestinal microbiome is culturable, complicating characterisation of the swine gastrointestinal ecosystem. The objective of this study was to determine the effect of a growth promotion dose (50g/ton) of chlortetracycline on the phylogenetic diversity of bacteria from swine faeces using a culture-independent method. Four freshly weaned pigs were provided a grower ration of primarily corn (63.7%) and soybean meal (25.2%) for 21 days; on Day 21 for 4 weeks the diet of two pigs was medicated with 50g/ton chlortetracycline. Faecal material was collected from each pig on Days 0, 14, 23, 28, 35, 42 and 49 for 454-pyrosequencing of the 16S rRNA gene. UniFrac analysis of pyrosequencing data showed no significant difference in bacterial diversity based on diet and among pigs (P>0.05) fed the low-level dose of chlortetracycline. The most abundant phyla in both treatment groups were Firmicutes, Bacteroidetes, Proteobacteria and Spirochaetes. Higher concentrations of chlortetracycline (e.g. 200g/ton or 400g/ton) may be required to observe a shift in the gastrointestinal flora in swine faeces compared with the low-level dose in this study. Studies of broader scope are needed to understand thoroughly how growth-promoting antimicrobials influence the gut microflora and benefit food animal growth efficiency.

published proceedings

  • J Glob Antimicrob Resist

author list (cited authors)

  • Poole, T. L., Suchodolski, J. S., Callaway, T. R., Farrow, R. L., Loneragan, G. H., & Nisbet, D. J.

citation count

  • 13

complete list of authors

  • Poole, TL||Suchodolski, JS||Callaway, TR||Farrow, RL||Loneragan, GH||Nisbet, DJ

publication date

  • May 2013