Liver‐type fatty acid binding protein interacts with hepatocyte nuclear factor 4α Academic Article uri icon

abstract

  • Hepatocyte nuclear factor 4α (HNF4α) regulates liver type fatty acid binding protein (L-FABP) gene expression. Conversely as shown herein, L-FABP structurally and functionally also interacts with HNF4α. Fluorescence resonance energy transfer (FRET) between Cy3-HNF4α (donor) and Cy5-L-FABP (acceptor) as well as FRET microscopy detected L-FABP in close proximity (~80 Å) to HNF4α, binding with high affinity Kd ~250-300 nM. Circular dichroism (CD) determined that the HNF4α/L-FABP interaction altered protein secondary structure. Finally, L-FABP potentiated transactivation of HNF4α in COS7 cells. Taken together, these data suggest that L-FABP provides a signaling path to HNF4α activation in the nucleus.

altmetric score

  • 0.5

author list (cited authors)

  • McIntosh, A. L., Petrescu, A. D., Hostetler, H. A., Kier, A. B., & Schroeder, F.

citation count

  • 11

publication date

  • October 2013

publisher