Production and characterization of agonistic monoclonal antibodies against chicken CD40. Academic Article uri icon

abstract

  • CD40 is mainly expressed by professional antigen-presenting cells (APCs). Its ligand, CD40L, is transiently expressed on activated CD4(+) T-cells. CD40-CD40L interactions mediate T-cell help to APCs and provide crucial signals for affinity maturation and B-cell class switching. In mammals, agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming and expansion, increased antibody production and class switching. In this study, we describe agonistic anti-chicken CD40 mAb 2C5. This mAb detected CD40 on primary chicken B-cells and macrophages, DT40 B-cells, and HD11 macrophages, induced NO synthesis in HD11 macrophages, and stimulated DT40 B-cell proliferation. These observations demonstrated at least partial functional equivalence of 2C5 to chicken CD154. This mAb may therefore constitute a new tool to study the role of CD40 in the chicken immune system, and its agonistic effects suggest that it could also be used as an adjuvant.

published proceedings

  • Dev Comp Immunol

author list (cited authors)

  • Chen, C., Abi-Ghanem, D., Njongmeta, L., Bray, J., Mwangi, W., Waghela, S. D., ... Berghman, L. R

citation count

  • 7

complete list of authors

  • Chen, Chang-Hsin||Abi-Ghanem, Daad||Njongmeta, Leo||Bray, Jocelyn||Mwangi, Waithaka||Waghela, Suryakant D||McReynolds, Jackson L||Ing, Nancy H||Berghman, Luc R

publication date

  • July 2010