Activity-dependent labeling and manipulation of fentanyl-recruited striatal ensembles using ArcTRAP approach. Academic Article

abstract

• Understanding the memory substrates underlying substance abuse requires thepermanent tagging and manipulation of drug-recruited neural ensembles. Here, we present a protocol for activity-dependent labeling and chemogenetic manipulationof fentanyl-activated striatal ensembles using the ArcTRAP approach. We outline the necessary steps to breed ArcTRAP mice, prepare drugs and reagents, conduct behavioral training, and perform tagging and manipulation. This approach can be adapted to investigate drug-recruited ensembles in other brain regions, providing a versatile tool for exploring the neural mechanisms underlying addiction. For complete details on the use and execution of this protocol, please refer to Wang etal.1.

authors

• Wang, Jun

published proceedings

• STAR Protoc

altmetric score

• 0.25

author list (cited authors)

• Xie, X., Chen, R., Wang, X., Smith, L., & Wang, J.

citation count

• 0

complete list of authors

• Xie, Xueyi||Chen, Ruifeng||Wang, Xuehua||Smith, Laura||Wang, Jun

publication date

• June 2023

publisher

• Elsevier  Publisher

keywords

• Animals
• Behavior
• Fentanyl
• Mice
• Neostriatum
• Neuroscience
• Skin Neoplasms

Digital Object Identifier (DOI)

• 10.1016/j.xpro.2023.102369

start page

• 102369

end page

• 102369

volume

• 4

issue

• 3

URL

• http://dx.doi.org/10.1016/j.xpro.2023.102369