Cloned mouse cerebrovascular endothelial cells that maintain their differentiation markers for factor VIII, low density lipoprotein, and angiotensin-converting enzyme. Academic Article

abstract

• This communication describes a relatively novel cell culture technique for the isolation of cerebrovascular endothelial cells from three strains of inbred mice. Cerebrovascular endothelial cells were identified by their morphology, the presence of Factor VIII-related antigen and angiotensin-converting enzyme, and the uptake of acetylated low-density lipoprotein. Cloned cerebrovascular endothelial cells were found to maintain their differentiated state and diploid genotype through 15 serial passages. The morphology and growth characteristics of these cells were found to be altered when cultured on different extracellular matrices. The isolation and cloning methods described are simple and highly reproducible.

authors

• Welsh, Christabel Jane

published proceedings

• In Vitro Cell Dev Biol Anim

altmetric score

• 3

author list (cited authors)

• Sapatino, B. V., Welsh, C. J., Smith, C. A., Bebo, B. F., & Linthicum, D. S.

citation count

• 30

complete list of authors

• Sapatino, BV||Welsh, CJ||Smith, CA||Bebo, BF||Linthicum, DS

publication date

• December 1993

publisher

• Springer Nature  Publisher

published in

• In Vitro Cellular and Developmental Biology - Animal  Journal

keywords

• Animals
• Brain
• Cell Separation
• Clone Cells
• Endothelium, Vascular
• Factor VIII
• Karyotyping
• Lipoproteins, LDL
• Mice
• Mice, Inbred Strains
• Peptidyl-Dipeptidase A

PubMed Central ID

• 8167915

Digital Object Identifier (DOI)

• 10.1007/BF02634230

start page

• 923

end page

• 928

volume

• 29A

issue

• 12

URL

• http://dx.doi.org/10.1007/bf02634230