Whole Genome Bisulphite Sequencing Using the Illumina Hiseq X System Institutional Repository Document uri icon

abstract

  • AbstractThe Illumina HiSeq X platform has helped to reduce the cost of whole genome sequencing substantially, but its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and the elimination of a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.

altmetric score

  • 34.38

author list (cited authors)

  • Suzuki, M., Liao, W., Wos, F., Johnston, A. D., DeGrazia, J., Ishii, J., ... Greally, J. M.

citation count

  • 4

complete list of authors

  • Suzuki, M||Liao, W||Wos, F||Johnston, AD||DeGrazia, J||Ishii, J||Bloom, T||Zody, MC||Germer, S||Greally, JM

Book Title

  • bioRxiv

publication date

  • September 2017