Genome-wide DNA methylation analysis using massively parallel sequencing technologies. Academic Article uri icon


  • "Epigenetics" refers to a heritable change in transcriptional status without alteration in the primary nucleotide sequence. Epigenetics provides an extra layer of transcriptional control and plays a crucial role in normal development, as well as in pathological conditions. DNA methylation is one of the best known and well-studied epigenetic modifications. Genome-wide DNA methylation profiling has become recognized as a biologically and clinically important epigenomic assay. In this review, we discuss the strengths and weaknesses of the protocols for genome-wide DNA methylation profiling using massively parallel sequencing (MPS) techniques. We will also describe recently discovered DNA modifications, and the protocols to detect these modifications.

published proceedings

  • Semin Hematol

author list (cited authors)

  • Suzuki, M., & Greally, J. M.

citation count

  • 10

publication date

  • January 2013