Genome-wide DNA methylation analysis using massively parallel sequencing technologies. Academic Article

abstract

• "Epigenetics" refers to a heritable change in transcriptional status without alteration in the primary nucleotide sequence. Epigenetics provides an extra layer of transcriptional control and plays a crucial role in normal development, as well as in pathological conditions. DNA methylation is one of the best known and well-studied epigenetic modifications. Genome-wide DNA methylation profiling has become recognized as a biologically and clinically important epigenomic assay. In this review, we discuss the strengths and weaknesses of the protocols for genome-wide DNA methylation profiling using massively parallel sequencing (MPS) techniques. We will also describe recently discovered DNA modifications, and the protocols to detect these modifications.

authors

• Suzuki, Masako

published proceedings

• Semin Hematol

author list (cited authors)

• Suzuki, M., & Greally, J. M.

citation count

• 10

publication date

• January 2013

publisher

• Elsevier  Publisher

keywords

• Animals
• DNA
• DNA Fingerprinting
• DNA Methylation
• Epigenesis, Genetic
• Genome
• High-Throughput Nucleotide Sequencing
• Humans

Digital Object Identifier (DOI)

• 10.1053/j.seminhematol.2013.01.002

start page

• 70

end page

• 77

volume

• 50

issue

• 1

URL

• http://dx.doi.org/10.1053/j.seminhematol.2013.01.002